Author Affiliations
Abstract
1 Department of Biomedical Engineering School of Medicine, Tsinghua University Beijing 100084, P. R. China
2 Center for Biomedical Imaging Research Tsinghua University Beijing 100084, P. R. China
A compact volume holographic imaging (VHI) method that can detect fluorescence objects located in diffusive medium in spectral selective imaging manner is presented. The enlargement of lateral field of view of the VHI system is realized by using broadband illumination and demagni fication optics. Each target spectrum of fluorescence emitting from a diffusive medium is probed by tuning the inclination angle of the transmission volume holographic grating (VHG). With the use of the single transmission VHG, fluorescence images with different spectrum are obtained sequentially and precise three-dimensional (3D) information of deep fluorescent objects located in a diffusive medium can be reconstructed from these images. The results of phantom experiments demonstrate that two fluorescent objects with a sub-millimeter distance can be resolved by spectral selective imaging.
Volume holographic grating diffusive medium fluorescence molecular imaging Journal of Innovative Optical Health Sciences
2016, 9(2): 1650010
Author Affiliations
Abstract
1 Department of Biomedical Engineering, Tsinghua University, Beijing 100084, China
2 Center for Biomedical Imaging Research, Tsinghua University, Beijing 100084, China
A reconstruction method guided by early-photon fluorescence yield tomography is proposed for time-domain fluorescence lifetime tomography (FLT) in this study. The method employs the early-arriving photons to reconstruct a fluorescence yield map, which is utilized as a priori information to reconstruct the FLT via all the photons along the temporal-point spread functions. Phantom experiments demonstrate that, compared with the method using all the photons for reconstruction of fluorescence yield and lifetime maps, the proposed method can achieve higher spatial resolution and reduced crosstalk between different targets without sacrificing the quantification accuracy of lifetime and contrast between heterogeneous targets.
170.3880 Medical and biological imaging 170.3010 Image reconstruction techniques 170.6960 Tomography 170.6920 Time-resolved imaging Chinese Optics Letters
2016, 14(7): 071702
